Epigenetic changes of mesenchymal stem cells in three-dimensional (3D) spheroids
نویسندگان
چکیده
Mesenchymal stem cells (MSCs) hold profound promise in tissue repair/regeneration. However, MSCs undergo remarkable spontaneous differentiation and aging during monolayer culture expansion. In this study, we found that 2-3 days of three-dimensional (3D) spheroid culture of human MSCs (hMSCs) that had been expanded in monolayer for six passages increased their clonogenicity and differentiation potency to neuronal cells. Moreover, in accordance with these changes, the expression levels of miRNA which were involved in stem cell potency were changed and levels of histone H3 acetylation in K9 in promoter regions of Oct4, Sox2 and Nanog were elevated. Our results indicate that spheroid culture increases their multi-potency and changes the epigenetic status of pluripotent genes in hMSCs.
منابع مشابه
The Effect of Rosmarinic Acid in Neural Differentiation of Wartons Jelly-derived Mesenchymal Stem Cells in Two Dimensional and Three Dimensional Cultures using Chitosan-based Hydrogel
Numerous studies have shown the positive effects of rosmarinic acid on the nervous system. Rosmarinic acid as a herbal compound with anti-inflammatory effects can prevent the destructive effect of inflammation on the nervous system. Furthermore, various studies have emphasized the advantages of three dimensional (3D) culture over the two dimensional (2D) culture of cells. In this study, thermos...
متن کاملScaffold Free 3D Culture of Mesenchymal Stem Cells; Implications for Regenerative Medicine
Mesenchymal stem cells (MSCs) are recognized as important sources for regenerative medicine (RM). Adherent cultures, long time considered definitory for MSCs phenotype, are relatively easy to perform and to assess. Two dimensional cell expansion systems, however, have been shown to decrease stemness and to induce cellular senescence therefore impeding on MSC therapeutic potential. Multicellular...
متن کاملIncreased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture
Mesenchymal stromal/stem cells (MSCs) have been investigated extensively through the past years, proving to have great clinical therapeutic potential. In vitro cultivation of MSCs in three-dimensional (3D) culture systems, such as scaffolds, hydrogels, or spheroids, have recently gained attention for tissue engineering applications. Studies on MSC spheroids demonstrated that such cultivation in...
متن کاملEvaluation of Changes in Global DNA Methylation during Osteoblastic Differentiation of Mesenchymal Stem Cells: A Laboratory Study
Background and Objectives: Control processes in osteoblastic differentiation of mesenchymal stem cells are not yet fully understood. Epigenetic mechanisms, especially the methylation of CpG Islands in the promoter of genes, are considered as one of the most important control mechanisms in stem cell differentiation. In the process of differentiation, it is debated whether only the methylation of...
متن کاملTissue Engineered Scaffolds in Regenerative Medicine
Stem cells are self-renewing cells that can be differentiated into other cell types. Conventional in vitro models for studying stem cells differentiation are usually preformed in two-dimensional (2D) cultures. The design of three-dimensional (3D) in vitro models which ideally are supposed to mimic the in vivo stem cells microenvironment is potentially useful for inducing stem cell derived tissu...
متن کامل